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Only homozygous animals ended up Utilized in the analyses, even when heterozygous mice were being run in behavioral experiments. A few cohorts of 11–sixteen mice underwent the first battery of behavioral assays for a complete of 42 animals. All mice had been to begin with housed with their littermates of exactly the same intercourse within the Vanderbilt Murine Neurobehavioral Core, with managed 12 h light/dim cycles, freely readily available meals and drinking water, and controlled temperature and humidity. The experimenter was initially blinded into the genotype of every animal, and each animal underwent exactly the same battery of behavioral checks to ascertain phenotype.
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